LOCUS       V012706                 5891 bp    DNA     circular SYN 08-APR-2021
DEFINITION  Exported.
ACCESSION   V012706
VERSION     V012706
KEYWORDS    pSilencer adeno 1.0-CMV
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   16..118
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     206..356
                     /label="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        447..750
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        751..954
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             1397..1816
                     /gene="IX"
                     /label="Hexon-interlacing protein"
                     /note="Hexon-interlacing protein from Human adenovirus C
                     serotype 5. Accession#: P03281"
     CDS             complement(1882..3216)
                     /gene="IVa2"
                     /label="Packaging protein 1"
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
     rep_origin      complement(4069..4657)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4831..5688)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5689..5793)
                     /label="AmpR promoter"
ORIGIN
        1 aattaattaa gctagcatca tcaataatat accttatttt ggattgaagc caatatgata
       61 atgagggggt ggagtttgtg acgtggcgcg gggcgtggga acggggcggg tgacgtagta
      121 gtgtggcgga agtgtgatgt tgcaagtgtg gcggaacaca tgtaagcgac ggatgtggca
      181 aaagtgacgt ttttggtgtg cgccggtgta cacaggaagt gacaattttc gcgcggtttt
      241 aggcggatgt tgtagtaaat ttgggcgtaa ccgagtaaga tttggccatt ttcgcgggaa
      301 aactgaataa gaggaagtga aatctgaata attttgtgtt actcatagcg cgtaatattt
      361 gtctagggag atccggtacc agatcttagt tattaatagt aatcaattac ggggtcatta
      421 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      481 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      541 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      601 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      661 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      721 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      781 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      841 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      901 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta
      961 gtgaaccgtc tcgagactac cgttgttata ggtgttcaag agacacctat aacaacggta
     1021 gtttttttac tagtaataaa ggatccttta ttttcattgg atccgtgtgt tggttttttg
     1081 tgtgcggccg cgtcgactct agtccccgcg gtggcagatc tggaaggtgc tgaggtacga
     1141 tgagacccgc accaggtgca gaccctgcga gtgtggcggt aaacatatta ggaaccagcc
     1201 tgtgatgctg gatgtgaccg aggagctgag gcccgatcac ttggtgctgg cctgcacccg
     1261 cgctgagttt ggctctagcg atgaagatac agattgaggt actgaaatgt gtgggcgtgg
     1321 cttaagggtg ggaaagaata tataaggtgg gggtcttatg tagttttgta tctgttttgc
     1381 agcagccgcc gccgccatga gcaccaactc gtttgatgga agcattgtga gctcatattt
     1441 gacaacgcgc atgcccccat gggccggggt gcgtcagaat gtgatgggct ccagcattga
     1501 tggtcgcccc gtcctgcccg caaactctac taccttgacc tacgagaccg tgtctggaac
     1561 gccgttggag actgcagcct ccgccgccgc ttcagccgct gcagccaccg cccgcgggat
     1621 tgtgactgac tttgctttcc tgagcccgct tgcaagcagt gcagcttccc gttcatccgc
     1681 ccgcgatgac aagttgacgg ctcttttggc acaattggat tctttgaccc gggaacttaa
     1741 tgtcgtttct cagcagctgt tggatctgcg ccagcaggtt tctgccctga aggcttcctc
     1801 ccctcccaat gcggtttaaa acataaataa aaaaccagac tctgtttgga tttggatcaa
     1861 gcaagtgtct tgctgtcttt atttaggggt tttgcgcgcg cggtaggccc gggaccagcg
     1921 gtctcggtcg ttgagggtcc tgtgtatttt ttccaggacg tggtaaaggt gactctggat
     1981 gttcagatac atgggcataa gcccgtctct ggggtggagg tagcaccact gcagagcttc
     2041 atgctgcggg gtggtgttgt agatgatcca gtcgtagcag gagcgctggg cgtggtgcct
     2101 aaaaatgtct ttcagtagca agctgattgc caggggcagg cccttggtgt aagtgtttac
     2161 aaagcggtta agctgggatg ggtgcatacg tggggatatg agatgcatct tggactgtat
     2221 ttttaggttg gctatgttcc cagccatatc cctccgggga ttcatgttgt gcagaaccac
     2281 cagcacagtg tatccggtgc acttgggaaa tttgtcatgt agcttagaag gaaatgcgtg
     2341 gaagaacttg gagacgccct tgtgacctcc aagattttcc atgcattcgt ccataatgat
     2401 ggcaatgggc ccacgggcgg cggcctgggc gaagatattt ctgggatcac taacgtcata
     2461 gttgtgttcc aggatgagat cgtcataggc catttttaca aagcgcgggc ggagggtgcc
     2521 agactgcggt ataatggttc catccggccc aggggcgtag ttaccctcac agatttgcat
     2581 ttcccacgct ttgagttcag atggggggat catgtctacc tgcggggcga tgaagaaaac
     2641 ggtttccggg gtaggggaga tcagctggga agaaagcagg ttcctgagca gctgcgactt
     2701 accgcagccg gtgggcccgt aaatcacacc tattaccggc tgcaactggt agttaagaga
     2761 gctgcagctg ccgtcatccc tgagcagggg ggccacttcg ttaagcatgt ccctgactcg
     2821 catgttttcc ctgaccaaat ccgccagaag gcgctcgccg cccagcgata gcagttcttg
     2881 caaggaagca aagtttttca acggtttgag accgtccgcc gtaggcatgc ttttgagcgt
     2941 ttgaccaagc agttccaggc ggtcccacag ctcggtcacc tgctctacgg catctcgatc
     3001 cagcatatct cctcgtttcg cgggttgggg cggctttcgc tgtacggcag tagtcggtgc
     3061 tcgtccagac gggccagggt catgtctttc cacgggcgca gggtcctcgt cagcgtagtc
     3121 tgggtcacgg tgaaggggtg cgctccgggc tgcgcgctgg ccagggtgcg cttgaggctg
     3181 gtcctgctgg tgctgaagcg ctgccggtct tcgccctgcg cgtcggccag gtagcatttg
     3241 accatggtgt catagtccag cccctccgcg gcgtggccct tggcgcgcag cttgcccttg
     3301 gaggaggcgc cgcacgaggg gcagtgcaga cttttgaggg cgtagagctt gggcgcgaga
     3361 aataccgatt ccggggagta ggcatccgcg ccgcaggccc cgcagacggt ctcgcattcc
     3421 acgagccagg tgagctctgg ccgttcgggg tcaaaaacca ggtttccccc atgctttttg
     3481 atgcgtttct tacctctggt ttccatgagc cggtgtccac gctcggtgac gaaaaggctg
     3541 tccgtgtccc cgtatacaga cttgagaggc ctgtcctcga ccgatgccct tgagagcctt
     3601 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
     3661 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg
     3721 cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat
     3781 cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa
     3841 gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc
     3901 gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat
     3961 gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca
     4021 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4081 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4141 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4201 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4261 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4321 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4381 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4441 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4501 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     4561 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4621 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     4681 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     4741 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     4801 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     4861 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     4921 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     4981 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     5041 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     5101 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc
     5161 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     5221 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     5281 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     5341 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     5401 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc
     5461 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     5521 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     5581 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     5641 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     5701 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     5761 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     5821 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     5881 ctttcgtctt c
//
